ABSTRACT
Prostate cancer is the most prevalent cancer found in men above the age of fifty years and is frequently diagnosed in men between 45 and 89 years of age with a median age of 72 years. This work was undertaken to assess oxidative stress and antioxidant status in patients with carcinoma of prostate. Erythrocyte ascorbic acid and plasma vitamin E levels were estimated in patients with carcinoma of prostate and compared to controls. It was observed that there was a significant decrease in Erythrocyte ascorbic acid and plasma vitamin E levels in patients with carcinoma of prostate compared to controls. The decrease in the levels of antioxidant vitamins may be due to the increased turnover for preventing the oxidative damage in these patients.
Subject(s)
Aged , Aged, 80 and over , Antioxidants/analysis , Ascorbic Acid/analysis , Erythrocytes/chemistry , Humans , Male , Middle Aged , Oxidative Stress , Prostatic Neoplasms/chemistry , Vitamin E/bloodABSTRACT
BACKGROUND: The exact pro-oxidant and antioxidant status in osteoarthritis patients is still not clear. To add a new insight to the question, changes in the erythrocyte lipid peroxidation products (MDA), levels of glutathione (GSH), ascorbic acid and plasma vitamin E (nonenzymatic antioxidant parameters); and activities of antioxidant enzymes superoxide dismutase (SOD), glutathione peroxidase (GPX), catalase in erythrocytes and plasma glutathione - S - transferase (GST) were measured in patients with osteoarthritis. AIM: This work was undertaken to assess oxidative stress and antioxidant status in patients with osteoarthritis. SETTINGS AND DESIGN: The study was conducted in 20 patients and compared to controls. Levels of erythrocyte MDA, GSH, ascorbic acid, plasma vitamin E; and activities of antioxidant enzymes were measured in patients with osteoarthritis. MATERIALS AND METHODS: Erythrocyte GSH was measured by the method of Beutler et al. Ascorbic acid levels were measured by the method of Tietz. Plasma vitamin E levels were measured by the method of Baker et al. MDA was determined as the measure of thio barbituric acid reactive substances (TBARS). SOD activity in the hemolysate was measured by the method of Misra and Fridovich. Activity of catalase was measured by the method of Beers and Sizer. GPX activity was measured as described by Paglia and Valentine in erythrocytes and Plasma GST activity was measured as described by Warholm et al. These parameters were measured in 20 patients and compared to controls. STATISTICAL ANALYSIS: Statistical analysis between group 1 (controls) and group 2 (patients) was performed by the student's t - test using the stat -view package. RESULTS: It was observed that there was a significant increase in erythrocyte MDA levels; SOD, GPX and plasma GST activities; and a significant decrease in erythrocyte GSH, ascorbic acid, plasma vitamin E levels and catalase activity in patients with osteoarthritis when compared to controls. CONCLUSIONS: The results of our study suggest higher oxygen-free radical production, evidenced by increased MDA and decreased GSH, ascorbic acid, vitamin E and catalase activity, support to the oxidative stress in osteoarthritis. The increased activities of antioxidant enzymes may be a compensatory regulation in response to increased oxidative stress.
Subject(s)
Adult , Antioxidants/metabolism , Ascorbic Acid/blood , Case-Control Studies , Female , Glutathione Peroxidase/blood , Glutathione Transferase , Humans , Lipid Peroxidation , Male , Middle Aged , Osteoarthritis/enzymology , Oxidative Stress , Superoxide Dismutase/blood , Vitamin E/bloodABSTRACT
Prostate cancer is the most prevalent cancer found in men above the age of fifty years and is frequently diagnosed in men between 45 and 89 years of age with a median age of 72 years. This work was undertaken to assess oxidative stress and anti oxidant status in patients with carcinoma of prostate. Glutathione (GSH), Malondialdehyde (MDA), Super Oxide Dismutase (SOD) levels in Erythrocytes and plasma Glutathione-S-Transferase (GST) levels were estimated in patients with carcinoma of prostate and compared to controls. It was observed that Erythrocyte GSH levels were significantly lower and Erythrocyte MDA & SOD levels were significantly higher in patients with carcinoma of prostate compared to controls. No significant change was observed in case of GST compared to controls. Oxidative stress may be involved in prostate cancer as evidenced by the higher MDA levels and lower GSH levels. The increased activity of antioxidant enzyme may be a compensatory regulation in response to oxidative stress.